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Literature summary for 2.3.1.297 extracted from
- 2-Hydroxy-ceramide synthesis by ceramide synthase family enzymatic basis for the preference of FA chain length (2008), J. Lipid Res., 49, 2356-2364 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CERS2, recombinant expression in HEK-293T cells, quantitative real-time quantitative PCR enzyme expression analysis | Homo sapiens |
| gene CERS4, recombinant expression in HEK-293T cells, quantitative real-time quantitative PCR enzyme expression analysis | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | in HeLa cells overproducing the fatty acid 2-hydroxylase FA2H, knockdown of CerS2 by siRNA results in a reduction in total long-chain 2-hydroxy-ceramides, confirming enzyme substrate specificity for chain length. Profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
| additional information | profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphingosine + behenoyl-CoA | Homo sapiens | - |
N-behenoylsphingosine + CoA | - |
? |  |
| sphingosine + eicosanoyl-CoA | Homo sapiens | - |
N-eicosanoylsphingosine + CoA | - |
? | |
| sphingosine + lignoceroyl-CoA | Homo sapiens | - |
N-lignoceroylsphingosine + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q96G23 | - |
- |
| Homo sapiens | Q9HA82 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| keratinocyte | epidermal, expression analysis of the CerS family members during keratinocyte differentiation, overview. A slight increase in CerS4 mRNA is observed at the late stage of differentiation | Homo sapiens | - |
| keratinocyte | epidermal, expression analysis of the CerS family members during keratinocyte differentiation, overview. Expression of CerS2 mRNA remains nearly unchanged during differentiation | Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | substrate specificity, overview | Homo sapiens | ? | - |
? | |
| additional information | substrate specificity, overview. Long-chain preference of CerS2 | Homo sapiens | ? | - |
? | |
| sphingosine + 2-hydroxybehenoyl-CoA | - |
Homo sapiens | N-2-hydroxybehenoylsphingosine + CoA | - |
? | |
| sphingosine + 2-hydroxyeicosanoyl-CoA | - |
Homo sapiens | N-2-hydroxyeicosanoylsphingosine + CoA | - |
? | |
| sphingosine + 2-hydroxylignoceroyl-CoA | - |
Homo sapiens | N-2-hydroxylignoceroylsphingosine + CoA | - |
? | |
| sphingosine + behenoyl-CoA | - |
Homo sapiens | N-behenoylsphingosine + CoA | - |
? | |
| sphingosine + eicosanoyl-CoA | - |
Homo sapiens | N-eicosanoylsphingosine + CoA | - |
? | |
| sphingosine + lignoceroyl-CoA | - |
Homo sapiens | N-lignoceroylsphingosine + CoA | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ceramide synthase 2 | - |
Homo sapiens |
| ceramide synthase 4 | - |
Homo sapiens |
| CerS2 | - |
Homo sapiens |
| CerS4 | - |
Homo sapiens |
| Lass2 | - |
Homo sapiens |
| Lass4 | - |
Homo sapiens |
| longevity assurance homologue 2 | - |
Homo sapiens |
| longevity assurance homologue 4 | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Homo sapiens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | in HeLa cells overproducing the fatty acid 2-hydroxylase FA2H, knockdown of CerS2 results in a reduction in total long-chain 2-hydroxy-ceramides, confirming enzyme substrate specificity for chain length. Profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
| malfunction | profiles of non-hydroxylated and 2-hydroxy-ceramides in transgenic HeLa cells expressing different CerS isozymes and or different specific interfence RNAs, overview | Homo sapiens |
| physiological function | differences in the expression patterns of CerS family members may play an important role in the production of the CER/2-hydroxy ceramide (CER) compositions of different chain lengths observed in different cell types and even in the altered production that occurring during keratinocyte differentiation | Homo sapiens |
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